EC-QC Laser Mid-IR Transmission Spectroscopy for Monitoring Beta-Aggregation in Alcohol-Denaturated Proteins

SCHWAIGHOFER, ANDREAS; ALCARAZ, MIRTA R.; KRISTAMENT, CHRISTIAN; GOICOECHEA, HÉCTOR C.; LENDL, BERNHARD

Viena

Conferencia; ICAVS 8 - International Conference on Advanced Vibrational Spectroscopy; 2015

TUWien

A rugged setup for mid-IR transmission measurements at large optical paths using an EC-QCL was employed for monitoring beta-aggregation in alcohol-denaturated proteins. Exposure of beta-rich a-chymotrypsin to 2,2,2-trifluoroethanol leads to immediate formation of nonnative alpha-helices. The subsequent gradual transition to intermolecular beta-sheet aggregates was monitored by mid-IR laser transmission spectroscopy. Multivariate curve resolution (MCR) was employed for analysis of spectral and concentration profiles of the temporal transition between alpha-helices an intermolecular beta-sheets.