A micellar-enhanced fluorescence photoinduced four-way calibration method for the determination of multiclass pesticides in lemon juice

ANTONIO, MARINA; ALCARAZ, MIRTA R.; FALCONE, R. DARIO; CULZONI, MARÍA J.

ANALYTICA CHIMICA ACTA

ELSEVIER SCIENCE BV

Año: 2023 vol. 1279 p. 341778 - 341789

0003-2670

In this work, a four-way multivariate calibration method for the simultaneous determination of four pesticides - carbendazim (CBZ), thiabendazole (TBZ), pirimiphos-methyl (PMM), and clothianidin (CLT) - in lemon juice is presented. Third-order data were acquired by registering the photoinduced fluorescence of the analytes as excitation-emission matrices at different times of UV-light irradiation, in the presence of organized media (direct micelles) as fluorescence enhancers. The optimal experimental conditions (pH 11.5 and 32 mmol L−1 hexadecyltrimethylammonium chloride surfactant) were determined through a central composite design using the response surface methodology. The analytes were individually calibrated, except for TBZ and CBZ due to the inner filter effect of TBZ on CBZ. Test samples containing all analytes and imidacloprid (as potential interference) were analysed. PARAFAC was utilized to evaluate both the trilinearity and quadrilinearity of the third-order data and four-way arrays, respectively. PMM was successfully determined with quadrilinear PARAFAC decomposition, whereas CLT, TBZ, and CBZ were satisfactorily modelled using U-PLS/RTL due to the loss of quadrilinearity caused by different phenomena. The profitable applicability of the analytical method in the CBZ, TBZ, PMM, and CLT determination in lemon juice samples was demonstrated, achieving limits of detection below the maximum residue levels reported by the European Commission, and mean recoveries at 90 ± 5%.