Garlic virus B (GarV-B) AND Garlic virus X (GarV-X): THE SAME VIRUS

CELLI M G; PEROTTO M.C.; TORRICO, A.K.; CONCI V. C.

Nigde

Simposio; 7th International Symposium on Edible Alliaceae; 2015

ISHS

Garlic virus B (GarV-B) AND Garlic virus X (GarV-X): THE SAME VIRUSM.G. Celli1,2, M.C. Perotto1,2, A.K. Torrico1, V.C. Conci1,2,*1Instituto de Patología Vegetal (IPAVE - CIAP) INTA; Camino. 60 cuadras Km 5,5 (5119) Córdoba,Argentina, phone 0054-351-4973636/4974343; 2CONICET. *conci.vilma@inta.gob.arGarlic is infected by a complex of viruses composed Potyvirus, Carlavirus andAllexivirus. The aim of this work was to identify and characterize new viruses infectinggarlic in Argentina. In 2013 samples were analyzed garlic (cultivar Mesidrome) byimmunosorbent electronmicroscopy and decoration with antisera pull: Onion yellowdwarf virus, Leek yellow stripe virus, Garlic common latent virus, Shallot latent virus,Garlic virus C and Garlic virus A. Were observed a decorated viral particles and otherundecorated. Total RNA were extracted and sent for sequencing (Illumina HiSeq 1500).The contigs were compared by Blastn with the GenBank database and showed thepresence of a contig (8336 nucleotides, nt) with 98% identity (59% coverage) with thelongest sequence GarV-B known to date (JN019813, 4923nt). The identity of nt andamino acid (aa) of the gene encoding the coat protein (CP) with others GarV-B isolateswas calculated using Lasergene 8.0.2 software package (DNASTAR, Inc., Madison,WI, USA) and showed higher values of 88.4% and 94.7%, respectively. According tothe International Committee on Taxonomy of Viruses (ICTV) criteria for differentiationof Allexivirus species (more than about 72% nt identity or 80% aa identity betweentheir CP or polymerase gene are the same species), the contig was considered the firstcomplete sequence of GarV-B (NC_025789/KM379144). The identity (nt and aa) of thepolymerase and CP available sequences of GarV-B with the others Allexiviruspublished in GenBank were calculated. The identity found was higher than thoseestablished by the ICTV in all comparisons (polymerase and CP) among isolates GarVBand GarV-X. These results suggest that GarV-B and GarV-X are the same viruses.Keywords: Garlic, Allium, Allexivirus.Work supported by INTA and CONICET.