Biological and molecular characterization of Garlic virus D and its effects on yields of garlic

CELLI M G; PEROTTO M C; BURASCHI D;; CONCI V. C

ACTA HORTICULTURAE

A.F. Gokce

Lugar: Nigde; Año: 2016 vol. 1 p. 193 - 200

0567-7572

AbstractGarlic is an agamic crop naturally infected by a viral complex includingPotyvirus, Carlavirus and Allexivirus which reduces its yield. The current work aimwas to characterize GarV-D biologically and molecularly and estimate its effect ongarlic yields. Virion modal lengths were calculated by comparison of 136 viralparticles and estimated in 734 nm. Total RNA from a sample was extracted and sentfor sequencing (HiSeq 1500 Illumina). The contig comparison based on GenBankshowed the presence of a 8,418 nucleotides (nt) sequence corresponding to a GarV-Disolate. The comparison of replicase and coat protein gen with other GarV-D isolatesshowed 83.4 to 93.3% and 86.5 to 89.1% nt identity respectively. Yield of virus-freegarlic, garlic only infected with GarV-D and plants infected with a viral mixture wasevaluated in assays under anti aphid cages and in field. At the end of the growth cycle,significant differences in weight and cloves number of garlic bulbs cultivated in fieldwere observed. The garlic infected with GarV-D isolate showed a reduction of 12.3%of weight, 6.7% of bulb caliber and 40.2% of number of cloves when compared withhealthy garlics. Some plants of the virus-free and GarV-D isolated treatmentsmaintained in the field showed infections with other viruses (OYDV, LYSV) which couldhave affected their yield. This situation may indicate that when GarV-D is isolated, noyield damage is produced, however when combined with others (as it happened withplants in field) synergic effect occurs and damage is more significant.