Beneficial effects of Saccharomyces cerevisiae RC016 in weaned piglets: in vivo and ex vivo analysis

GARCÍA G. R.; DOGI, C. A.; POLONI V. L.; FOCHESATO A. S.; DE MORENO DE LEBLANC A.; COSSALTER A. M. ; PAYROS D.; OSWALD I. P. ; CAVAGLIERI, L.R.

Beneficial Microbes

Wageningen Academic Publishers

Año: 2019 vol. 10 p. 33 - 42

1876-2883

Probiotics have been explored to replace antibiotics as growth promoters in animal feed. Theyrepresent a potential safe advance to control enteric bacterial diseases and to improve gut immunity. Saccharomyces cerevisiae RC016 was previously isolated from gut pig and showed in vitro beneficial properties (inhibition and coagregation of pathogens) and mycotoxin adsorbent.abilities (aflatoxin B1; Ochratoxin A and zearalenone). The aim of this work was to evaluate beneficial properties of S. cerevisiaeRC016in a non-inflammatoryin vivo model in weaned piglets and in an intestinal inflammation ex vivomodelinduced by the mycotoxin deoxynivalenol. Secretory IgA (s-IgA) levels, intestinal cytokines, goblet cellsand production parameters were evaluated in a pig model.For the in vivo assays, a total of six pigs were weaned at 21 days and assigned to in two groups: Control Group (n=6) and Yeast Group (n=6). Animals received yeast strain for three weeks. After 22 days small intestine was recovered for determination of goblet cells and s-IgA.Forthe ex vivo assay, jejunal explants were obtained from 5 weeks old crossbred piglets and treated as follow 1) control, 2) treated for 3 h to with 10 μM deoxynivalenol (DON) used as an inflammatory stressor, 3) incubated with 107CFU/ml yeast strain, 4)pre-incubated 1 h with 107CFU/ml yeast strainand then treated for 3 h to with 10 μM DON. CCL20, IL-1β, IL-8 and IL-22 gene expressionwas determined byqPCR. Oral administration of S. cerevisiae RC016 increaseds-IgA, the number of goblet cells in small intestine and all the growth parameters measured. In the ex vivo model, the cytokine profile studied showed a potential anti-inflammatory effect of the administration of the yeast. In conclusion, S. cerevisiae RC016 is a promising candidate for feed additives formulation to improve animal growth and gut immune system. This yeast strain could be able to improve the gut health through counteractingthe weaning-associated intestinal inflammation in piglets.