Effect of plant hormones on trichome initiation and secondary metabolism in Solanum sp

INES SCHAUVINHOLD (ABATEDAGA), KATRIN BESSER, STEVE SLOCOMBE AND PIERRE BROUN

Reading, Reino Unido

Conferencia; 4ta Conferencia Internacional de Metabolomica en Plantas; 2006

Solanum sp. have up to seven different anatomical types of trichomes. They can be subdivided into glandular and non-glandular types. Glandular types have been shown to contain several secondary metab- olites. We are investigating the relationship between the presence and density of these glands with the production of some of these compounds such as terpenoids and acylsugars. In S. esculentum, it was found that glandular type VI produces and stores mono and sesquiterpenoids. The composition of this mixture in each variety varies in quality and quantity. We also found a second possible glandular type not described in S. esculentum before, similar to types IV from S. hirsutum. We are currently testing its pro- duction of secondary metabolites against the known compounds that are produced by these glands in the other species, i.e., S. pennellii type IVs produce ac- ylsugars. Using GC–MS and LC–MS to study the production of secondary metabolites will allow us to describe secondary metabolite production in these glands in different developmental stages and in response to plant hormones. The main purpose is to study how trichome initia- tion (for each type of trichome) and the production of secondary metabolites (terpenes and/or acylsugars) are influenced by gibberellins (GA) and jasmonates (JA), and the interaction between these two hormones. Our preliminary results show that the non-glandular type V is up regulated by GA in the same way than Arabid- opsis trichomes (non-glandular). Glandular type VI initiation, which has been described to respond to JA, is not up regulated by GA. We are currently studying the levels of mono and sesquiterpenoids in GA treat- ments. Also we are setting metabolites baselines to study the interaction of JA and GA in trichome sec- ondary metabolism and initiation using tomato GA mutants (pro and gib-1).