ORAL ADMINISTRAION OF IMMUNOMODULATORY BACTERIUM LIKE PARTICLES ENHANCES MUCOSAL AND SYSTEMIC IMMUNE RESPONSE INDUCED BY ROTAVIRUS ORAL VACCINE

RAIMONDO, M.P.; RAYA-TONETTI, M.F.; SALVA, S.; ALVAREZ, S.; VILLENA, J.; VIZOSO PINTO, M. G.

San Miguel de Tucumán

Simposio; SIBAL 2016_ V International Symposium on Lactic Acid Bacteria; 2016

CERELA - CONICET

Rotavirus (RV) is the major causative agent of gastroenteritis in children under 5 years, being a major cause of mortality. There are two oral commercial vaccines for preventing RV infection: Rotarix (monovalent) and RotaTeq (pentavalent). RV vaccines have been included into the compulsory vaccination schedule in several countries including Argentina. In order to enhance effectiveness and eventually reduce costs of RV vaccination, we investigated the adjuvant capacity of Immunomodulatory Bacterium Like Particles (IBLP) obtained by acid-heat treatment of Lactobacillus rhamnosus CRL1505, L. rhamnosus IBL027, L. plantarum CRL1506, or L. paraplantarum CRL1905 co-administered with Rotateq in a murine model. Three-week old Balb/c mice were orally immunized three times every 14 days with 4x104 infecting units/mouse of RotaTeq vaccine (control) or RotaTeq administered with the different IBLP (1x108 particles/mice). Fifteen days after the last immunization, the humoral and cellular immune responses at the mucosal and systemic compartments were evaluated. Orally administered RV vaccine was able to induce humoral and cellular specific immune responses as evidenced by the detection of specific intestinal and serum IgG, IgA and IgM as well as increased production of IL-4 and IFN-γ by splenocytes and lymphocytes from Peyer´s patches after antigen stimulation. The four IBLPs evaluated in this work were able to increase the levels of specific IgA in intestinal fluid (p