KIDNEY MODIFICATIONS IN TWO HYPERTENSIVE MODELS. ROLE OF NITRIC OXIDE

CHABAN HASSAN; LLICAS SANTIAGO; MAMANI IRINA; NAVARRO ALVARO; JOO TURONI, CLAUDIO; PERAL DE BRUNO M

Tucumán

Jornada; 3ª Reunión Conjunta de Sociedades de Biología de la República Argentina; 2015

The kidney is involved in the regulation of arterial hypertension (HT). In rats, the inhibition of nitric oxide (NO) by Nω-Nitro-Largininemethyl ester (LR) and the hydrosaline retention by deoxycorticosterone acetate (DR) produce HT of different etiology.However, the protective role of renal NO in these models has not been studied at all. Objective: To evaluate renal histologicalchanges in LR and DR and the protective role of NO in each model. Methods: Creatinine (CrC), Na+ (Na+C) clearence and meanarterial pressure were determined in LR and DR. In one kidney, corpuscular, tubular (TA) and vascular (VA) areas, and Bowman?sspace (BS) were measured by histological techniques. In the other kidney, the bioavailability of NO was measured in cortex andmedulla by Griess reaction. Results were compared to control rats (CR).Results: LR and DR developed HT without macroscopic renal changes or CrC alteration. Plasma Na+ increased in LR and DR. Na+Calso increased but only in DR. A reduction in corpuscular area and BS was found in LR and DR. TA decreased more in DR than RL,and VA decreased only in RL. Nitrites were lower in renal cortex (CR: 2.7±0.4 vs LR: 0.6±0.1 pmol/mg of tissue; p