INVESTIGADORES
DRAGHI Walter Omar
artículos
Título:
First genomic analysis of the broad-host-range Rhizobium sp. LPU83 strain, a member of the low-genetic diversity Oregon-like Rhizobium sp. group
Autor/es:
TORRES TEJERIZO, G.A.; DEL PAPA, M.F.; DRAGHI, W.O.; LOZANO, M.J.; GIUSTI, M.A.; MARTINI, C.; SALAS, M.E.; SALTO, I.; WIBBERG, D.; SZCZEPANOWSKI, R,; WEIDNER, S; SCHLÜTER, A.; LAGARES, A.; PISTORIO, M.
Revista:
JOURNAL OF BIOTECHNOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2011 vol. 155 p. 3 - 10
ISSN:
0168-1656
Resumen:
Alfalfa (Medicago sativa) is the most cultivated forage legume for
cattle and animal feeding, occupying about 32 million hectares over the
world. Management of the N₂-fixing symbiosis of this plant to maximize
crop production is therefore an important objective. A fundamental
constraint to this aim emerges when a moderately low soil pH hampers the
establishment of an effective symbiosis with indigenous and/or
inoculated rhizobia. Besides the association of alfalfa with Ensifer
(Sinorhizobium) meliloti, this legume is able to establish a symbiosis
with Ensifer (Sinorhizobium) medicae and with less characterized types
of rhizobia, such as the Oregon-like strains, Rhizobium sp. Or191
initially isolated in the USA, and the Rhizobium sp. LPU83 strain, from
Argentina. These strains are acid-tolerant, highly competitive for
acidic-soil-alfalfa nodulation, but inefficient for biological nitrogen
fixation with alfalfa. These features position the Oregon-like rhizobia
as strains of potential risk in agricultural soils compared with the
efficient symbiont E. meliloti. Moreover, the collected genetic
information has revealed that the genomic structure of these rhizobial
isolates is complex in terms of sequence similarities shared with other
rhizobia. Such a "patched" genetic composition has obviously imposed
severe restrictions to the classical taxonomy of these rhizobia. In this
work we summarize the accumulated knowledge about the Oregon-like
rhizobia and present a phylogenetic analysis based on genome sequence
data of Rhizobium sp. LPU83 obtained by a high-throughput sequencing on
the Genome Sequencer FLX Titanium platform. The accessibility of the
complete genomic sequence will release up more experimental
possibilities since this information will then enable biochemical
studies as well as proteomics and transcriptomics approaches.