Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils

MARTINA, PABLO; LEGUIZAMON, MARIANA; PRIETO, CLAUDIA I.; SOUSA, SILVIA A.; MONTANARO, PATRICIA; DRAGHI, WALTER O.; STÄMMLER, MAREN; BETTIOL, MARISA; DE CARVALHO, CARLA C. C. R.; PALAU, JULIANA; FIGOLI, CECILIA; ALVAREZ, FLORENCIA; BENETTI, SILVINA; LEJONA, SERGIO; VESCINA, CECILIA; FERRERAS, JULIÁN; LASCH, PETER; LAGARES, ANTONIO; ZORREGUIETA, ANGELES; LEITÃO, JORGE H.; YANTORNO, OSVALDO M.; BOSCH, ALEJANDRA

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY.

SOC GENERAL MICROBIOLOGY

Año: 2018

1466-5026

Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis(CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-yearepidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and fromhospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by morethan 3 % from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), theseisolates clustered with the agricultural soil strain, Burkholderia sp. PBP 78, which was already deposited in the PubMLSTdatabase. The isolates were examined using a polyphasic approach, which included 16S rRNA, recA, Matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities(ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the sevenisolates represent a single novel species within the Bcc, for which the name Burkholderia puraquae sp. nov. is proposed.Burkholderia puraquae sp. nov. CAMPA 1040T (=LMG 29660T=DSM 103137T) was designated the type strain of the novelspecies, which can be differentiated from other species of the Bcc mainly from recA gene sequence analysis, MLSA, ANIb,MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 C, aesculin hydrolysis, and lysinedecarboxylase and b-galactosidase activities.