Osmotic fragility in glucose-treated or diabetic erythocytes ismediated by acetylated tubulin

NIGRA A ; MONESTEROLO N; AMAIDEN MR; RIVELLI J; CASALE C; SANTANDER V.

Rosario, Santa Fe

Congreso; X Reunión Anual de la Sociedad Argentina de Investigación de Bioquímica y Biología Molecular (SAIB).; 2014

In previous work we have observed by experiments in vivo and in vitro of analysis erythrocytes from diabetic subjects suggest that high glucose concentration induces translocation of tubulin from sedimentable fraction to the plasma membrane in erythrocytes and this change affected cellular deformability. In addition, glucose increased level acetylated tubulin and its translocation to the membrane. In this work osmotic fragility of erythrocytes treated with high glucose concentrations was analyzed. Thus, we observed a significant increase of erythrocyte osmotic fragility in diabetic individuals and similar results by incubation of erythrocytes with glucose control subjects. Then the effect of the enzyme deacetylases inhibition was evaluated. The erythrocytes pretreated with trichostatin A and then incubated for 3 hours with glucose have an increased hemolysis compared to those that were only with glucose. Following acetylation capacity was analyzed in each case. The results show that significantly reduces the capacity of acetylation in diabetics erythrocytes or exposed to glucose, which was corroborated by treating erythrocytes with tubacin as a specific inhibitor of HDAC6. This work allows us to conclude that the increased level of acetylated tubulin in erythrocyte membrane affects its properties and that this increase occurs in diabetics by the decrease in acetylation capacity.