BoHV-1 subtypes circulating in Argentina

MAIDANA, S.; LOMONACO, M.; COMBESSIES, G.; CIANFRINI, D.; PALÁCIOS M. DE LOS A.; ROMERA, S.A.

Santiago. Chile.

Congreso; XXVI World Buiatrics Congress 2010; 2010

XXVI World Buiatrics Congress c/o Kenes Latin America – Global Congress Organizers and Association Management Services

Infectious bovine rhinotracheitis, infectious pustular vulvovaginitis and infectious pustular balanopostitis are the major clinical syndromes caused by bovine herpesvirus 1 (BoHV-1) in domestic and wild cattle. Classified as a list B disease by the Office International des Epizooties, BoHV-1 is distribute worldwide and is endemic in Argentina, where it is responsible for important economic losses due to the decreased production. In Argentina, seroprevalence rates averaged 60% of cattle (between 24.8 and 84.1% by region and animal ages). Several studies based on restriction analysis and the use of monoclonal antibodies allowed the description of three BoHV-1subtypes (BoHV-1.1, BoHV-1.2a and BoHV-1.2b). We proposed to analyze the circulating of field isolates of BoHV-1 in Argentina since 1984 until now. Restriction endonuclease analysis (REA) was performed to 10 isolates from the strain bank of INTA Castelar. Most samples were obtained from animals with respiratory symptoms compatible with Herpesvirus, although there were some cases with nervous sings. The isolates were amplified in MDBK cells. After viral DNA was extracted and confirmed the identity by PCR. The DNA was digested with the HindIII enzyme, run on a 0.7% agarose gel stained with ethidium bromide and the banding pattern was observed with UV light. Five of the 10 isolates tested showed a restriction profile similar to the reference strain BoHV-1.1 (Los Angeles strain) and the other corresponding to a similar pattern to the reference strain BoHV-1.2 (K22 strain). These data constitute a contribution to the characterization of BoHV-1 strains circulating in Argentina for the rational use of vaccines.