Isolation of a paramyxovirus of Argentinean water buffaloes

MAIDANA, S.; LOMONACO, M.; RODRIGUEZ, D.; PALACIOS, M.A.; CRUDELLI, G.; ROMERA, S.A.

Santiago. Chile.

Congreso; XXVI World Buiatrics Congress 2010; 2010

XXVI World Buiatrics Congress, c/o Kenes Latin America – Global Congress Organizers and Association Management Services

In the last few decades, many novel paramyxovirus have emerged causing catastrophic illnesses in different species, and some of them jumped from those species to humans. Parainfluenza virus type 3 is considered to be the clinically most important virus among the parainfluenza viruses and represents one of the most common respiratory disease agents. Bovine parainfluenza type 3(BPIV3) is a respiratory pathogen associated with bronchopneumonia in cattle. It is known their synergistic action with bacteria and other related viruses in the productions of pneumonic diseases. In the present study we report the first isolate of PI3 from Argentinean buffaloes. Nasal and genital swabs buffalos with respiratory disease and abortions were evaluated. The virus was isolated in MDBK cell culture and characterized by electron microscopy, indirect immunofluorescence assay (IFA), haemagglutinatiuon assay (HA) and haemoadsorption assays. The serum of affected animals was evaluated by haemagglutination inhibition with BPIV3 reference strain. Transmission electron microscopy revealed spherical to pleomorfic virions approximately 50-300 nm in diameter morphologically similar to paramyxoviruses. Bovine anti-BPIV3 serum was able to detect the cells infected with this virus by IFA and haemoadsorption test. The isolate was able to agglutinate to guinea pigs red blood cells (16 UHA) and to sticking them onto monolayer infected. The antibody titer of the infected animal was 1.6 (log 1/40). In Adittion, 50 percent of the animals in the herd were seropositive to BPIV3. Our results show the circulation of PI3 in Argentinean buffaloes. In the future it is necessary to determine the exact identity of the isolates by molecular studies.