Electrophysiological and behavioural response of Aedes albopictus to n-heinecosane, an ovipositional pheromone of Aedes aegypti

PAULA V. GONZALEZ; PAOLA GONZÁLEZ AUDINO; HECTOR MASUH

ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2014 p. 1 - 7

0013-8703

Aedes aegypti (L.) and Aedes albopictus (Skuse) (Diptera: Culicidae) are highly anthropophilic mos- quito species and potential vectors of dengue and yellow fever. The location of suitable sites for ovipo- sition requires a set of visual, tactile, and olfactory cues that influence females before they lay their eggs. In this study, the effect of n-heneicosane, a recognized oviposition pheromone of Ae. aegypti, on the olfactory receptors of the antennae of Ae. aegypti and Ae. albopictus was studied using electroan- tennographic detection coupled to gas chromatography (GC-EAD). A significant electroantenno- graphic response to n-heneicosane in adult females of both mosquito species was observed. In addition, gravid Ae. albopictus females laid more eggs in substrate treated with n-heneicosane at 0.1, 1, or 10 p.p.m. than in the control, denoting oviposition attractancy. Conversely, at 30, 50, 100, and 200 p.p.m., more eggs were laid in the control substrate, indicating oviposition repellency. Analysis of the larval cuticle by GC and mass spectrometry confirmed the presence of n-heneicosane in the cuticles of Ae. albopictus larvae. The species-specific role of n-heneicosane as an oviposition pheromone in Ae. aegypti and its significance as a behaviour modifier of Ae. albopictus in breeding sites is discussed.