CHOLINERGIC SYSTEM MODULATES ACTIVATION OF NEUTROPHIL GRANULOCYTES FROM GLIOBLASTOMA PATIENTS

SAIBENE, P; INFANTE, A; CORONEL, JV; MAIUMI, S; JANCIC, C; ROSSATO, P; ITURRIZAGA, J; VERMEULEN, M; VILLAVERDE M. S.; TREVANI, P; SALAMONE, G

Congreso; SAIC 2022; 2022

Glioblastoma multiforme (GBM) is the deadliest and most common type of human primary brain tumor in adults, with a mean survival of less than a year after diagnosis. This tumor is defined by hallmark features, such as a great proliferative capacity, diffuse infiltration in adjacent tissues, necrotic focus and increased angiogenesis. Acetylcholine (ACh) is a neurotransmitter from ganglionic and post-ganglionic parasympathetic nerves and a non-neuronal paracrine mediator produced by different cell types. It has been established that ACh exceeds its role as a neurotransmitter, being involved in a broad variety of non-neuronal systems, including the immune system and tumor progression. Polymorphonuclear (PMN) were observed in large numbers in necrotic areas, these infiltrating cells could promote the expansion of the glioma stem cell pool and may play an important role in glioma pathology. The aim of this work is to evaluate the relevance of the cholinergic system in human PMN obtained from healthy donors (PMN-h) and glioblastoma patients (PMN-p). Both PMN-h and PMN-p were isolated from peripheral blood by Ficoll–Paque gradient centrifugation and dextran sedimentation. PMN were incubated with agonist cholinergic for 18 h and the supernatants were collected, and cytokine were evaluated by ELISA. CD11b expression was evaluated by flow cytometry after activation by cholinergic system by 15 min. An increase in the expression of CD11b (p