Dabrafenib resistance promotes anchorage-independent cell population growth and mesenchymal markers increase in BRAFV600E melanoma cells.

ARBE, MARÍA FLORENCIA; ASPARCH, YAMILA; GLIKIN, GC; FINNOCHIARO, LME; VILLAVERDE, MS

Congreso; SAIC 2022; 2022

Epithelial-mesenchymal transition (EMT) has a driving role in migration and invasion promoting the acquisition of metastatic potential by tumor cells. In this process, epithelial cells lose their cell contact, rearrange their cytoskeleton, acquire a migratory phenotype, and downregulate E-cadherin (epithelial marker) and increase vimentin and N-cadherin (mesenchymal markers) expression. BRAFV600E mutation has been suggested to be involved in the development of EMT. Our group previously reported a cell contact loss in 3D spheroids and an increase in anchorage-independent cell population in A375 Dabrafenib-resistant cells (A375-R). In this work, we investigated the EMT marker expression (E-cadherin, N-cadherin, and vimentin) in adherent and anchorage-independent or suspension A375-R cells using western blot, flow cytometry, and immunofluorescent techniques. Suspension A375-R cells showed a downregulation of E-cadherin and up-regulation of vimentin (p