THYMIC STROMAL LYMPHOPOIETIN (TSLP) IS A NEUTROPHIL CELLS MODULATOR: RELEVANCE IN THE PATHOGENESIS OF BRAIN TUMORS

CORONEL, JV; ITURRIZAGA, J; INFANTE, A; JANCIC, C; VEMEULEN, M; RABADAN, A; CANDOLFI, M; VILLAVERDE, MARCELA SOLANGE; LENIKOV, FR; SALAMONE, G

Congreso; SAIC 2020; 2020

Background: Glioblastoma (GBM) is the most devastating brain tumor, with an associated poor prognosis. Despite the advances insurgery and chemoradiation, the survival of GBM patients has notimproved significantly in the past three decades. Thymic stromallymphopoietin (TSLP) is a cytokine produced primarily by activatedepithelial cells and it has been shown to be a key factor in maintaining immune homeostasis and regulating inflammatory responses atmucosal barriers. However, recent studies have found an expandingrole for TSLP in inflammatory diseases and cancer.This work aimed to elucidate the relevance of TSLP in the interaction between neutrophils and GBM cells. First, we evaluated theproduction of TSLP by tumor cells through RT-PCR and then, we determined whether the TSLP treatment affects the cross-talk betweenneutrophils and tumor GBM cells. For those purposes, human U251cell line or tumoral cells obtained from a GBM patient were co-cultured with human neutrophils. We observed that the U251 cell lineor the primary cell culture incubated with Epidermal Growth Factor(EGF) produced TSLP (p