Characterization and effects of gene/chemotherapy on new established cell lines derived from melanoma patients

FONDELLO, C; VILLAVERDE, MS; GLIKIN, GC; FINOCCHIARO, LME

Congreso; SAIB; 2014

The aim of the present study was to evaluate the in vitro effects of a combination of bleomycin and human interferon-B (hIFN-B) lipofection on cell lines derived from spontaneous human melanoma. Three new cells lines (MLUTG-1, MLUTG-2 and MLUTG-4) were established from surgically excised metastatic tumors by mechanical disruption and serial passages. The growth duplication times were respectively: 24.4 ± 0.6; 33.3 ± 0.4 and 59.4 ± 0.9 h, for MLUTG-1, MLUTG-2 and MLUTG-4. As determined by immunochemistry, the three cell lines presented the specific melanoma markers S-100, HMB-45 and Melan-A. The antitumor effects of hIFN-B lipofection in the absence or presence of bleomycin were evaluated by the acidic phosphatase assay (APH). Lipofection with hIFN-B significantly decreased cell viability (p=0.001) of MLUTG-1 and MLUTG-2 (36.5 ± 0.7% and 35 ± 5% respectively) and increased the concentration of intracellular oxidants in both cell lines (as determined by DCFH-DA assay). In the case of MLUTG-1, the combination of hIFN-B lipofection plus bleomycin significantly increased (p