Adverse effects promoted by a commercial formulation of the insecticide Fipronil in liver biomarkers of sábalos (Prochilodus lineatus)

LOCH, LAUREANO; SANTILLAN, ANTONELLA; ONDARZA, P.; MIGLIORANZA, K.; DE LA TORRE, F.R.

Buenos Airs

Congreso; SETAC Latin America 11th Biennial Meeting; 2015

SETAC Latinoamericana

>Current Use Pesticides (CUPs) are emerging pollutants of special concernbecause their increasing input into the aquatic environments. Fipronil is a CUPswhose toxicity in insects is well documented, although limited knowledgeconcerning sublethal effects is available in other non-target organisms (eg,fishes). In this context, the biomarker responses promoted in juveniles of P.lineatus were evaluated after 48 h of exposure to a sublethal concentration of acommercial formulation of fipronil (Fp) dissolved in water (nominal value of 100µg/L). The reversibility of these responses was also studied in exposedindividuals after 15 days of permanence in tapwater under laboratory conditions(RFp). A Control series of unexposed individuals were simultaneously testedduring exposure (Ctrl) and recovery (RCtrl) phases. Water samples were taken inorder to measure levels of toxic. After the experimental period, fish wereweighed, anesthetized and the liver were extracted. Condition factor (CF) andliver somatic index (LSI) were calculated, glutathione-S-transferase activity(GST) as well as the levels of lipid peroxidation by TBARS and antioxidantcapacity against peroxyl (ACAP) were determined. CYP1A protein expression inliver was also estimated by means Western blotting. Differences between groupswere analyzed using one way ANOVA and Tukey´s multiple comparisons. Nosignificant differences among groups were detected in CF, LSI or GST. Fishexposure to Fp promoted an increase of 61% in TBARS levels but also in ACAPvalues (65%) showing a decrease in the antioxidant capacity respect to Ctrl group.Fipronil exposure also enhanced the response of CYP1A and reactive bands wereobserved in Fp group. Transference of exposed individuals to control media(RFp) promoted a non significant decrease in TBARS values respect RCtrl group,however ACAP values differentiated between these two groups. These resultsindicated that a short term exposure of formulated fipronil promoted in liver ofthis native species adverse effects showing that biotransformation processes weremediated by CYP1A and that oxidative damage was generated on lipids. Assayedrecovery phase would also confirm the availability of reversing the adverseeffects promoted by Fipronil.