Pulse and Spiked Sediments Bioassay With a Commercial Formulation of the Insecticide Fipronil: Biomarker Responses in the Fish Prochilodus lineatus

LAGRAÑA, ALDANA; LOZANO, ISMAEL; MIGLIORANZA, K.; DE LA TORRE, F.R.

Santiago de chile

Congreso; SETAC Latin America 14th Biennial Meeting; 2021

Society of Environmental Toxicology and Chemistry (SETAC)

Toxicity of fipronil (FP) is well documented in insects although limited knowledge of sublethal effects is available on fish. Fipronil can often reach aquatic environments via local inputs or surface runoff. Environmental concentrations previously reported in sediments were up to 5.5 µg/kg. In this work, we evaluated biomarker responses in target organs of Prochilodus lineatus juveniles after exposure to a commercial formulation of FP (Clap Bayer®) in a sediment static bioassay. Fish (4.2 ± 0.9 g b.w; n=27) were exposed during 14 days to three experimental conditions: fish exposure to sediments spiked with Clap (Sp) (5.5 µg/kg); fish exposure to sediments with a contaminant pulse with Clap (Pu) (50 µg/l), and control sediments (Ctrl). Following exposure, liver, brain, gills and muscle were collected. Antioxidant defenses (catalase activity, CAT; glutathione-S-transferase activity, GST; antioxidant capacity against peroxyls, ACAP; reduced glutathione content GSH) and oxidative damage parameters (levels of lipid peroxidation by TBARS) were determined in liver, brain and gills. Brain-motor parameter (acetylcholinesterase activity (AChE)) was evaluated in brain and muscle. Sediments, water and suspended particulate matter were taken for analytical quantification of FP. Differences between groups were analyzed by ANOVA followed by Tukey test (p