INVESTIGADORES
MULLER IGAZ Lionel Ivan
congresos y reuniones científicas
Título:
ROLE OF TDP-43 IN POST-TRANSCRIPTIONAL REGULATION: IMPLICATIONS FOR NEURODEGENERATION AND PLASTICITY PROCESSES.
Autor/es:
LUCHELLI L; IGAZ LM
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; XXVIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN).; 2013
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias (SAN).
Resumen:
TDP-43 protein has been identified as the main proteic component of the
intracellular ubiquitinated inclusions in most cases of Amyotrophic Lateral
Sclerosis (ALS) and frontotemporal dementia (FTD), two neurodegenerative
processes that are encompassed by the term "TDP-43 proteinopathies". We
are studying in vivo the intrinsic
properties and physiological role of TDP-43 to define its involvement in
neurodegenerative disease. Consequently, we propose to study in vivo TDP-43/mRNA and TDP-43/protein interactions
in brain tissue from wild-type mice, with special emphasis on those genes involved
in neuronal plasticity and survival. To do this, we are applying different
techniques including single and double immunofluorescence, co-immunoprecipitation,
immunoblotting, RT-PCR and subcellular fractionation by gradient centrifugation.
To investigate the physiological role of TDP-43 and the contribution of TDP-43
in maintaining transcript and protein levels, we are using an antisense
oligonucleotide (ASO) approach to stereotaxically infuse TDP-43 or control ASOs
for region-specific knock-down of the protein. These studies will provide relevant
information to understand the physiological functions of TDP-43 and the molecular mechanisms involved in the pathogenesis
of TDP-43 proteinopathies and related neurodegenerative diseases, which in turn
may provide the basis for new therapeutic approaches.