INVESTIGADORES
NORES Maria Jimena
artículos
Título:
ORF-C4 from the early branching eukaryote Giardia lamblia displays characteristics of α-crystallin small heat shock proteins.
Autor/es:
NORES, M J., PRUCCA, C.G., QUIROGA, R., ELíAS, E.V., CAVALLíN, L., PRICE, A.M, SAURA, A., CARRANZA, P.G., GOTTIG, N., SOLARI A.J. AND LUJAN, H.D.
Revista:
BIOSCIENCE REPORTS
Editorial:
PORTLAND PRESS LTD
Referencias:
Año: 2009 vol. 29 p. 25 - 34
ISSN:
0144-8463
Resumen:
Giardia lamblia is a medically important protozoan parasite with a basal position in the eukaryotic lineage and
is an interesting model to explain the evolution of biochemical events in eukaryotic cells. G. lamblia trophozoites
undergo significant changes in order to survive outside the intestine of their host by differentiating into infective
cysts. In the present study, we characterize the previously identified Orf-C4 (G. lamblia open reading frame C4) gene,
which is considered to be specific to G. lamblia. It encodes a 22 kDa protein that assembles into high-molecularmass
complexes during the entire life cycle of the parasite. ORF-C4 localizes to the cytoplasm of trophozoites and
cysts, and forms large spherical aggregates when overexpressed. ORF-C4 overexpression and down-regulation do
not affect trophozoite viability; however, differentiation into cysts is slightly delayed when the expression of ORFC4
is down-regulated. In addition, ORF-C4 protein expression is modified under specific stress-inducing conditions.
Neither orthologous proteins nor conserved domains are found in databases by conventional sequence analysis of the
predicted protein. However, ORF-C4 contains a region which is similar structurally to the á-crystallin domain of sHsps
(small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which
is involved in the response to stress (including encystation) in G. lamblia.is a medically important protozoan parasite with a basal position in the eukaryotic lineage and
is an interesting model to explain the evolution of biochemical events in eukaryotic cells. G. lamblia trophozoites
undergo significant changes in order to survive outside the intestine of their host by differentiating into infective
cysts. In the present study, we characterize the previously identified Orf-C4 (G. lamblia open reading frame C4) gene,
which is considered to be specific to G. lamblia. It encodes a 22 kDa protein that assembles into high-molecularmass
complexes during the entire life cycle of the parasite. ORF-C4 localizes to the cytoplasm of trophozoites and
cysts, and forms large spherical aggregates when overexpressed. ORF-C4 overexpression and down-regulation do
not affect trophozoite viability; however, differentiation into cysts is slightly delayed when the expression of ORFC4
is down-regulated. In addition, ORF-C4 protein expression is modified under specific stress-inducing conditions.
Neither orthologous proteins nor conserved domains are found in databases by conventional sequence analysis of the
predicted protein. However, ORF-C4 contains a region which is similar structurally to the á-crystallin domain of sHsps
(small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which
is involved in the response to stress (including encystation) in G. lamblia.G. lamblia trophozoites
undergo significant changes in order to survive outside the intestine of their host by differentiating into infective
cysts. In the present study, we characterize the previously identified Orf-C4 (G. lamblia open reading frame C4) gene,
which is considered to be specific to G. lamblia. It encodes a 22 kDa protein that assembles into high-molecularmass
complexes during the entire life cycle of the parasite. ORF-C4 localizes to the cytoplasm of trophozoites and
cysts, and forms large spherical aggregates when overexpressed. ORF-C4 overexpression and down-regulation do
not affect trophozoite viability; however, differentiation into cysts is slightly delayed when the expression of ORFC4
is down-regulated. In addition, ORF-C4 protein expression is modified under specific stress-inducing conditions.
Neither orthologous proteins nor conserved domains are found in databases by conventional sequence analysis of the
predicted protein. However, ORF-C4 contains a region which is similar structurally to the á-crystallin domain of sHsps
(small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which
is involved in the response to stress (including encystation) in G. lamblia.Orf-C4 (G. lamblia open reading frame C4) gene,
which is considered to be specific to G. lamblia. It encodes a 22 kDa protein that assembles into high-molecularmass
complexes during the entire life cycle of the parasite. ORF-C4 localizes to the cytoplasm of trophozoites and
cysts, and forms large spherical aggregates when overexpressed. ORF-C4 overexpression and down-regulation do
not affect trophozoite viability; however, differentiation into cysts is slightly delayed when the expression of ORFC4
is down-regulated. In addition, ORF-C4 protein expression is modified under specific stress-inducing conditions.
Neither orthologous proteins nor conserved domains are found in databases by conventional sequence analysis of the
predicted protein. However, ORF-C4 contains a region which is similar structurally to the á-crystallin domain of sHsps
(small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which
is involved in the response to stress (including encystation) in G. lamblia.G. lamblia. It encodes a 22 kDa protein that assembles into high-molecularmass
complexes during the entire life cycle of the parasite. ORF-C4 localizes to the cytoplasm of trophozoites and
cysts, and forms large spherical aggregates when overexpressed. ORF-C4 overexpression and down-regulation do
not affect trophozoite viability; however, differentiation into cysts is slightly delayed when the expression of ORFC4
is down-regulated. In addition, ORF-C4 protein expression is modified under specific stress-inducing conditions.
Neither orthologous proteins nor conserved domains are found in databases by conventional sequence analysis of the
predicted protein. However, ORF-C4 contains a region which is similar structurally to the á-crystallin domain of sHsps
(small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which
is involved in the response to stress (including encystation) in G. lamblia.á-crystallin domain of sHsps
(small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which
is involved in the response to stress (including encystation) in G. lamblia.G. lamblia.