Exogenous Nitro-Oleic Acid Treatment Inhibits Primary Root Growth by Reducing the Mitosis in the Meristem in Arabidopsis thaliana

DI FINO, LUCIANO M.; CERRUDO, IGNACIO; SALVATORE, SONIA R.; SCHOPFER, FRANCISCO J.; GARCÍA-MATA, CARLOS; LAXALT, ANA M.

Frontiers in Plant Science

frontiers

Año: 2020 vol. 11

Nitric oxide (NO) is a second messenger that regulates a broad range of physiologicalprocesses in plants. NO-derived molecules called reactive nitrogen species (RNS) canreact with unsaturated fatty acids generating nitrated fatty acids (NO2-FA). NO2-FA workas signaling molecules in mammals where production and targets have been describedunder different stress conditions. Recently, NO2-FAs were detected in plants, howevertheir role(s) on plant physiological processes is still poorly known. Although in this workNO2-OA has not been detected in any Arabidopsis seedling tissue, here we show thatexogenous application of nitro-oleic acid (NO2-OA) inhibits Arabidopsis primary rootgrowth; this inhibition is not likely due to nitric oxide (NO) production or impaired auxin orcytokinin root responses. Deep analyses showed that roots incubated with NO2-OA had alower cell number in the division area. Although this NO2-FA did not affect the hormonalsignaling mechanisms maintaining the stem cell niche, plants incubated with NO2-OAshowed a reduction of cell division in the meristematic area. Therefore, this work showsthat the exogenous application of NO2-OA inhibits mitotic processes subsequentlyreducing primary root growth.