EXOTOXINS HLYA AND SHLA CONTROL CYTOTOXIC PHENOTYPES OF TARGET EUKARYOTIC CELLS THROUGH PURINERGIC SIGNALING

MARISEL TUTTOBENE; MARIA FLORENCIA LEAL DENIS; CORA L. ÁLVAREZ; JULIETA SCHACHTER; NICOLAS A. SAFFIOTI; NATALIA LAURI; MARIANO A. OSTUNI; VANESA HERLAX; ELEONORA GARCÍA- VÉSCOVI; PABLO SCHWARZBAUM

Congreso; Reunion Anual de sociedades de Biociencias; 2023

Aim: we characterized the effects of exotoxins from gram(-) bacteria, i.e., HlyA from uropathogenic strains of Escherichia coli and ShlA from Serratia marcescens, on eukaryotic cell models. Focus was given to the capacity of these toxins to induce intracellular ATP release from target cells, and consequent purinergic signaling. Human erythrocytes (RBCs) were exposed to HlyA, while CHO (chinese hamster ovary) cells were exposed to ShlA. Methods: luciferin-luciferase luminometry was used to quantify extracellular ATP (eATP). Electrical impedance was used to quantify cell volume of RBCs. Autophagy in CHO cells was revealed by an EGFP-LC3 green fluorescent punctate pattern. Significance level at P