Morphological traits and high resolution RAPD markers for the identification of the main strawberry varieties cultivated in Argentina

GARCIA, M.G.; ONTIVERO, M.I.; JUAN C. DIAZ RICCI; CASTAGNARO, A.P:

PLANT BREEDING

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Berlin; Año: 2002 vol. 121 p. 76 - 80

0179-9541

Eight genotypes of the main Fragaria ´ ananassa cultivars grown in Argentina were analysed using the random ampli®ed polymorphic DNA (RAPD) technique combined with electro-phoresis in polyacrylamide gels. The high resolution of this procedure allowed the detection, with only 13 random primers, of 37 genotype-speci®c bands that can be used as markers for verifying the identity of cultivars. By using this approach, three di€erent accessions of the cultivar `Pa jaro´ exhibited di€erences in ampli®cation pro®les, con®rming the need for DNA analysis to prevent misidenti®cation of cultivars. In addition, RAPD bands and morphological traits were used to assess genetic relatedness among cultivars. Comparison of both dendrograms revealed that there is no correlation between the clustering obtained with molecular and morphological characters.