INVESTIGADORES
GONZALEZ CASTRO Mariano
artículos
Título:
DNA barcoding Neotropical fishes: recent advances from the Pampa Plain, Argentina
Autor/es:
ROSSO, J. J., E. MABRAGAÑA, M. GONZÁLEZ CASTRO & J. M. DÍAZ DE ASTARLOA.
Revista:
MOLECULAR ECOLOGY RESOURCES
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2012 p. 999 - 1011
ISSN:
1755-098X
Resumen:
The fish fauna of the Pampa Plain, the southernmost distribution range of many Neotropical species, was barcoded
in this study. COI sequences were analysed by means of distance (K2P/NJ) and character-based (ML) models, as well
as the Barcode Index Number (BIN). K2P/NJ analysis was able to discriminate among all previously identified species
while also revealing the likely occurrence of two cryptic species that were further supported by BIN and ML
analyses. On the other hand, both BIN and ML were not able to discriminate between two species of Rineloricaria.
Despite the small genetic divergence between A. cf. pampa and A. eigenmanniorum, a tight array of haplotypes was
observed for each species in both the distance and character-based methods. Deep intraspecific divergences were
detected in Cnesterodon decemmaculatus (5%) and Salminus brasiliensis (6%). For Salminus brasiliensis, these findings
were further supported by character-based (ML) evidence and meristic and morphological data. Our results also
showed that Pampa Plain representatives of Salminus brasiliensis, Rhamdia quelen, Hoplias malabaricus, Synbranchus
marmoratus, Australoheros facetus, Oligosarcus jenynsii and Corydoras paleatus differed by more than 3% from
their conspecifics from other parts of South America. Overall, this study was able to highlight the likely occurrence
of a cryptic species in Salminus brasiliensis and also illustrate the strong geographical structure in the COI sequence
composition of seven fish species from South America.
Keywords: Argentina, biodiversity assessment, DNA barcoding, fish species, Salminus brasiliensis