In vitro effects of 1a,25(OH)2D3-glycosides from Solbone A (Solanum glaucophyllum leaves extract; Herbonis AG) compared to synthetic 1a,25(OH)2D3 on myogenesis

VALERIA GILI; VERONICA GONZALEZ PARDO; ANA CAROLINA RONDA; PABLO DE GENERARO; HEINI BACHMANN; RICARDO BOLAND; ANA RUSSO DE BOLAND

STEROIDS

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2016

0039-128X

The presence of glycoside derivatives of 1a,25(OH)2D3 endows plants to gradual release of the free bioactive form of 1a,25(OH)2D3 from its glycoconjugates by endogenous animal tissue glycosidases. This results in increased half-life of the hormone in blood when purified plant fractions are administered for therapeutic purposes. In this work, we evaluated the role 1a,25(OH)2D3-glycosides enriched natural product (Solbone A) from Solanum glaucophyllum leaf extract compared with synthetic 1a,25(OH)2D3 on myogenic differentiation in C2C12 myoblasts. For these, differentiation markers and myogenic parameterswere studied in C2C12 myoblasts. Results showed that Solbone A, likewise the synthetic hormone, increased creatine kinase activity at day 2 after differentiation induction (60%, p < 0.05). Solbone A and synthetic 1a,25(OH)2D3 increased vitamin D3 receptor protein expression at 10 nM (50% and 30%, respectively) and the transcription factor myogenin (80%, p < 0.05). However, tropomyosin expression was not affected by both compounds. In addition, myosin heavy chain (MHC) protein expression was increased 30% at day 2 of differentiation. Solbone A or synthetic 1a,25(OH)2D3 had no effects on myogenin nor MHC cell localization. Cellular mass increased with myogenesis progression, being Solbone A more effective than synthetic 1a,25(OH)2D3. Finally, Solbone A, as well as synthetic 1a,25(OH)2D3, augmented the index fusion of cultured muscle fibers. In conclusion, these results demonstrated that Solbone A exhibit atleast equal or greater effects on early myoblast differentiation as synthetic hormone, suggesting that plant glycosides could be an effective, accessible and cheaper substitute for synthetic 1a,25(OH)2D3 to promote muscle growth.