MOLECULAR STUDIES OF GLYCEROL-3-PHOSPHATE DEHYDROGENASE FROM TRIATOMA INFESTANS THORACIC MUSCLES

STROPPA, M. M., SORIA, N., CARRIAZO, C., PEREIRA, R., GEREZ DE BURGOS, N.M.

Rosario - Argentina

Congreso; Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2006

MOLECULAR STUDIES OF GLYCEROL-3-PHOSPHATE DEHYDROGENASE FROM TR <!-- @page { size: 21cm 29.7cm; margin: 2cm } P { margin-bottom: 0.21cm } --> Triatoma infestans (T. infestans), Chagas disease vector, acquirers wings and the ability to fly after the last molt from fifth instar nymph to adult. The ability to fly is important for dispersion. In previous studies, in our laboratory, it was demonstrated that glycerol-3-phosphate dehydrogenase (GPDH), involved at glycerophosphate shuttle, increase its activity 30 fold in adults thoracic muscles. Adults muscles should have higher glycolitic and respiratory capacity to support fly activity. Electrophoretic studies from thoracic muscles showed two GPDH isoenzymes. Nymphs predominant isoform has less mobility. The aim of this work is to begin GPDH molecular studies. Using cDNA pools with degenerated primers, we amplified a 200 bp RT-PCR product from 1 to 3 days old T. infestans adults thoracic muscles. Upon sequencing and database alignments the fragment show to be part of GPDH cDNA. Based on the sequence obtained, specific primers were designed for RACE experiment. We successfully amplified the 5´ and 3´end of the GPDH cDNA. Sequencing results allow identity, homology and conserved domains comparative studies with others species.