Daily variations of GPDH transcripts profile in Triatoma infestans flight muscles

STROPPA MM, LAGUNAS MS, CARRIAZO CS, GARCIA BA, VERMUOTH N, GEREZ DE BURGOS NM

Simposio; XII Latin American Symposium on Chronobiology; 2013

Triatoma infestans, main vector of Chagas disease, is a blood-sucking insect. Adult?s flight dispersal is the most important mechanism for reinfestation of houses after insecticide spraying. Flight muscles have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. In this work, we explored the profile of GPDH expression in females and males adult flight muscles under light/dark cycle (LD), constant light (LL) and constant dark (DD). Under DD conditions, GPDH-1 flight muscles of T. infestans showed a rhythmic pattern of transcription synchronic with a rhythmic profile of activity suggesting regulation by the endogenous circadian clock. Otherwise, the GPDH-2 expression analysis no revealed regulation by the endogenous clock, but instead an external factor such as the dark/light period showed to be necessary for the synchronization of GPDH-2 transcription and activity.