Sex, ingestion and temperature condition the expression of GPDH in T. infestans flight muscles

STROPPA M M; CARRIAZO C; GEREZ DE BURGOS N M

Hotel Portal del Lago,Villa Carlos Paz, Córdoba, Argentina

Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en; 2008

Triatoma infestans (T. infestans), Chagas´ disease vector, acquires wings and the ability to fly after the last molt from fifth instar nymph to adult.The ability to fly is important for insect dispersion.GPDH isozymes are differentially expressed during flight muscles development.The aim of this work is to begin GPDH transcripts expression studies during flight muscles development in both sexes and with different ingestion and room temperatures of 22 and 28 °C.GPDH transcripts patterns were studied by RT-PCR and the PCR-bands were semiquantified with a graphical method.The pattern of expression differs between sexes.The expression of GPDH-1 begins earlier in females and GPDH-2 is more abundantly expressed than in those from males.The expression pattern showed changes with increase of ingestion quantity.Before the last molt,GPDH-1 is 2 fold higher and GPDH-2 is 25 fold higher. In young adults,GPHD-1 decreased in 20 % and GPDH-2 increased in 40 %.In 30 days old adults,the GPDH-2 was 20 % higher and GPDH-1 had no significant modification.During flight muscles development the expression pattern was different at room temperatures of 22 and 28 °C.At 22 °C the pattern showed delayed changes than that observed at 28 °C.These results are consistent with the expression and the metabolic role described for GPDH isozymes in thoracic muscles of flying insects.