mARNs TRANSCRIPTS OF GLYCEROL-3-PHOSPHATE

STROPPA MARÍA M., GOTTIG NATALIA, CARRIAZO CARLOTA, PEREIRA

Bariloche - Río Negro

Congreso; Sociedad Argentina de Investigación Bioquímica y Biología Molecular, 40ª Reunión Anual; 2003

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

Triatoma infestans (T. infestans), vector of Chagas’s disease, acquire wings and the ability to fly, which is important for their dispersal, after the last molt from fifth instar nymph to adult. In previous studies, in our laboratory, we have demonstrated that glycerol-3-phosphate dehydrogenase (GPDH), enzyme involved in glycerophosphate shuttle, increases 30 fold its activity in adults thoracic muscles. Adult muscles would have higher glycolitic and respiratory capacity than those of fifth instar nymphs for flight. Two different isozymes forms were studied by electrophoresis from extracts of adults and nymphs thoracic muscles, showing a slower mobility the last one. In order to study the expression genetic regulation of GPDH we have started to study RNAs transcrips from adults muscles. We have extracted total RNA and realized RT-PCR with four degenerated primers. We have amplified in higher amount transcripts with molecular weighs about 1.0 Kb and 2.0 Kb. The transcript about 2 Kb seems to be a primary  transcript and the other, a mature transcript, may corresponds with adult enzyme form of GPDH. This transcripts were sequenced and compared with Data Bases on-line with GPDHs of other known flight insects muscles.