EXPRESSION OF GPDH ISOFORMS IN FLIGHT MUSCLE FROM WILD AND LABORATORY Triatoma infestans ADULTS

STROPPA MM, CARRIAZO C, LAGUNAS M, REYNOSO R, PEREIRA R, GEREZ DE BURGOS NM.

Puerto Madryn

Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2010

Triatoma infestans (T. infestans), vector of Chagas disease, acquires wings and develops flight ability after shedding the last nymphal stage to adult. The flight is important in the recolonization of the housing post fumigation. The enzyme glycerol-3-phosphate dehydrogenase (GPDH) is involved in providing energy for flight. In T. infestans, we characterized two isoforms of GPDH transcripts (GPDH-1 and GPDH-2). Previous studies showed that its expression in flight muscle varies during the development of flight ability, according to sex and to changes in temperature and time of intake. The aim of this study was to analyze the expression of the isoforms of GPDH in flight muscle of T. infestans adult from natural population and laboratory colonies. We were performed semiquantitative RT-PCR, non-denaturing PAGE electrophoresis revealed with specific enzyme activity and determination of enzymatic total activity. We found that GPDH-1 is the predominant isoform in flight muscle T. infestans adult natural population and laboratory colonies. In laboratory colonies, comparing the expression in the first with the second generation, there are an increase in the expression of GPDH-2 and a decrease in the expression of GPDH-1. This is consistent with the metabolic functions described for isoforms of GPDH and the adjustment to a regular supply of food.