Metabolic pathways in neutrophils in a model of early maternalplacental interaction

G CALO, D VOTA, FL SABBIONE, F MERECH, V HAUK, R RAMHORST, A TREVANI AND C PÉREZ LEIROS.

Congreso; Reunión Anual Conjunta SAIC-SAI-SAFIS; 2021

Trophoblast cells (Tb) interact with maternal immune cells at pla-centation favoring an anti-inflammatory microenvironment required for fetal growth. Circulating neutrophils (PMN) appear activated during pregnancy and even more in pregnancies complicated by preeclampsia. Metabolic regulation underlies the functional profiling of immune cells in a number of settings but immunometabolic repro-gramming in the context of pregnancy is still enigmatic.We have shown that conditioned media (CM) from human first tri-mester Tb (Swan-71 cell line) inhibit PMA-induced neutrophil ex-tracellular trap formation, promote PMN apoptosis and induce an angiogenic profile on PMN.The aim of this work was to explore the effect of trophoblast cell-derived factors in neutrophils? functional and metabolic profile.Neutrophils from healthy donors were cultured with Swan-71 condi-tioned media (CM)-2% FCS. PMN activation profiles were assessed by RT‐qPCR and flow cytometry. Treg modulation was explored co-culturing PMN with autologous mononuclear cells and CD4, FOXP3 staining. Glucose uptake and intracellular lipid accumulation were determined by flow cytometry using the glucose fluorescent analog 2-NBDG or BODIPY 493/503, respectively.PMN pre-incubated with CM increased the frequency of double stained CD4+ FOXP3+ cells in co-cultures, compared to 2% serum media (basal) (% X±SE Basal 3.85±0.64 CM 9.97±3.38, P