Exacerbated endoplasmic reticulum stress suffered by endometrial stromal cells alters the conditioning of maternal monocytes to a tolerogenic dendritic cell profile.

A SCHAFIR, L FERNÁNDEZ, E SOCZEWSKI, D VOTA, Y BESTACH, L CASTAGNOLA, J BLEJER, A GRASSI BASSINO, C PÉREZ LEIRÓS, S GORI AND R RAMHORST.

virtual

Congreso; Reunión Anual Conjunta SAIC-SAI-SAFIS; 2021

SAIc-SAI-SAFIS

Endometrial stromal cells undergo physiological endoplasmic reticu-lum stress (ERS) and unfolded protein response (UPR) allowing the secretion of immunoregulators and associated with a sterile inflam-matory response to achieve a successful implantation. However, an exacerbated ERS/UPR was found in endometria of women with recurrent pregnancy loss, suggesting that a rigorous balance is re-quired. Considering that stromal cells condition maternal monocytes to a tolerogenic dendritic cell profile, here we evaluated the impact of ERS/UPR triggered on stromal cells on monocyte differentiation. Thus, human endometrial stromal cell line (HESC) were treated: +/- Thapsigargin (an ERS-inducer Tg, 4h) or +/- decidualization stimuli (MPA+db-cAMP for 8 days). Conditioned media (CM) were collected after 48h. Then, isolated monocytes from peripheral blood mononu-clear cells from healthy women were cultured with rhGM-CSF+rhIL-4 for 5 days in the absence/presence of CM. Monocyte-derived cul-tures differentiated with HESC+Tg CM showed a lower frequency of CD1a+CD14- cells compared with HESC CM, quantified by FACS analysis (p