Trophoblast cells modulate the functional profile of dendritic cells

G. SALOMONE; L. FRACCAROLI; L. VOJACEK; C. PÉREZ LEIRÓS; J. GEFFNER; R. RAMHORST

Rio de Janeiro, Brasil

Congreso; 4th Brazilian Symposium on HLA and Disease/ 2nd Symposium on Reproductive immunology; 2009

Many evidence indicates a pivotal role of Dendritic cells (DCs) in shaping the cytokine profile, regulating tissue remodeling and angiogenesis towards a tolerogenic microenvironment at the maternal-fetal interface. Here, we investigate the modulation of the physiology of the DCs during the dialogue with trophoblast cells in the early stages of implantation. DCs were differentiated from peripheral blood monocytes (80% purity) from fertile women, with IL-4 and GM-CSF during 5 days. DCs were cocultured with trophoblast cells (Swan-71 cell line obtained from cytotrophoblast) as a model of the feto-maternal dialogue. After 24h the DCs were collected and incubated in the presence/absence of LPS 0.1g/ml for additional 24h. The phenotype analyzed by FACS revealed that DCs did not modulate the MIF (mid intensity fluorescence) of CD1, DR, CD86 and CD40. However, the DCs that interacted with trophoblast cells and were cultured with LPS during 24h, displayed a significant increase in the MIF of DR and a decrease in CD86. In addition, DCs cocultivated with trophoblast significantly increased the production of IL-10, MCP-1 and IL-6 in comparison with DCs in media (p