VIP contribution to the selective recruitment of Tregs during the peri-implantation period.

L. GALLINO, E. GRASSO , V. HAUK, D. VOTA, J. WASCHEK, C. PEREZ LEIRÓS AND R. RAMHORST

Buenos Aires

Congreso; Reunión Conjunta de Sociedades Biomédicas; 2017

Reunión Conjunta de Sociedades Biomédicas

Decidualization process involves phenotypic and functional changes on endometrial cells and the modulation of different mediators such as the secretion of the vasoactive intestinal peptide (VIP). This neuropeptide is a key regulator of the immunetolerance capable of inducing Tregs towards alloantigens which are crucial for a successful implantation and the pregnancy maintenance. VIP deficient(+/-) pregnant mice have reduced litter size and increased inter-gestations period. Here, we evaluated VIP contribution to the Tregs? recruitment during the peri-implantation period. We used two approaches: VIP(+/-) and Foxp3-knock-in-GFP females either pregnant or in estrous. Foxp3 expression by RT-PCR was not detectable in VIP(+/-) mice uterus, in contrast to Foxp3-GFP mice, confirmed by FACS analysis. This reduction was accompanied with a higher expression of RORγt and a decrease in IL-10 (p