VIP induces the decidualization program on human endometrial stromal cells and conditions dendritic cells profile

GRASSO E, GORI S, PAPARINI D, SALOMONE S, MARTINEZ G, PÉREZ LEIRÓS C, RAMHORST R

Congreso; LXII Reunión Científica anual de la Sociedad Argentina de Inmunología y LIX Reunión anual Sociedad Argentina de Investigación Clínica; 2014

SAI-SAIC

The decidualization program involves phenotype and funtional changes on endometrial cells that will facilitate the attachment and invasion of the blastocyst. This process involves the modulation of different mediators such as cytokines, chemokines and growth factors. Particularlly, the vasoactive intestinal peptide (VIP) is a neuropeptide produced by endometrial stromal cells among others, and displays multiple target circuits that allow immunetolerance. Here, we investigated VIP contribution to the decidualization program and whether this process modulates dendritic cells (DC) profile. We differenciated human endometrial stromal cell line (hESC) in the presence of VIP (10-7M) or with medroxiprogesterone (MPA) and dbcAMP (Positive control) during 8d. The decidualization performed with MPA+dbcAMP increased VIP expression (by RT-PCR) and secretion (by ELISA). When the decidualization was induced by VIP, we observed an increase IGFBP1, PRL and KLF13/KLF9 ratio in a concentration-dependent manner; accompained by and increase in the expression of IL-8 and SDF1, both markers of receptive endometrium (p