Modulation of maternal LIF producers T cells by trophoblast and paternal antigens

L. FRACCAROLI; E. GRASSO; E. ZEITLER; E. LOMBARDI; J. ETCHEPAREBORDA; C. NAGLE; M. CORTELEZZI; C. PÉREZ LEIRÓS; R. RAMHORST

Buenos Aires

Congreso; VI encuentro Latinoamericano de Ginecología Reproductiva y endocrinológica; 2010

SAEGRE

The allorecognition in uterus enhances the production of growth factors essentials for embryonic development, such as leukaemia inhibitory factor (LIF) meantime neuropeptides, as vasoactive intestinal peptide (VIP), and progesterone modulate the pro inflammatory response during the feto-maternal dialog. Here, we evaluated the contribution of maternal CD4 cells, activated by paternal or trophoblast antigens, to LIF production and its modulation by VIP and progesterone in fertile women or with recurrent spontaneous abortions (RSA). The frequency of fertile-CD4+LIF+ cells increased by VIP and progesterone after the co culture with paternal alloantigens or with an immortalized trophoblast cell line (Swan 71). This increase was accompanied by a decrease in MMP-9 gelatinolitic activity and an increase in the pSTAT3/STAT3 ratio. Although, under the same conditions, RSA patients have decreased levels of LIF expression and these could not be modulated by VIP and progesterone. Moreover, these patients have reduced the number of endometrial infiltrated CD4+LIF+ cells in comparison with fertile women and could be related with the exacerbated inflammatory response observed in the maternal-fetal dialogue model.