Cellular senescence association to endoplasmic reticulum stress in endometrial stromal cells

SOCZEWSKI E, MURRIETA-COXCA JM, P FUENTES ZACARIAS, M L GRASSO E, MARTÍ M, MORALES-PRIETO D, PÉREZ LEIRÓS C, MARKERT U, RAMHORST R

Congreso; IX SLIMP, Latin Amirican Society for materno fetal interaction and placenta; 2022

Objective: At decidualization, cells undergo endoplasmic reticular stress (ERS) and unfolded protein response (UPR), which will allow them to expand their endoplasmic reticulum with the corresponding machinery for protein folding. Here, we focus on the role of ERS/UPR during decidualization to induce a physiological sterile inflammatory response and whether it might be regulate by miRNAs. Methodology: We used an in vitro model of decidualization represented by human telomerase-immortalized endometrial stromal cell line St-T1b differentiated with 8-BrcAMP; and endometrial biopsies from patients with recurrent pregnancy loss (RPL) and recurrent in vitro fertilization failures (RIF). Results: First, we evaluated the ERS by the expression of ATF6α, PERK and IRE1α. Decidualized cells increased the expression of the three sensors (p