Trophoblast cells recruit maternal Monocytes and modulate the chemokines and their receptors expression-profile under pathological conditions

ESTEBAN GRASSO; LAURA FRACCAROLI; VANESA HAUK; FEDERICO BAUDOU; CLAUDIA PEREZ LEIROS; ROSANNA RAMHORST

Buenos Aires

Congreso; First French-Argentine Immunology Congress y LVIII Reunión Anual de la Sociedad Argentina de Inmunología.; 2010

Sociedad Argentina de Inmunología

Macrophages constitute 20-30% of decidual immune cells and they coordinate the defense against pathogens, wound healing and tolerance induction against paternal antigens. Here, we study whether the maternal monocytes (MO) are attracting toward the trophoblat cells under physiological and pathological conditions. First, we performed migration assay with 8um transwell system using maternal PBMC in the upper compartment and trophoblat cells (Swan-71 cells line) in the lower compartment. The FACS analysis showed that trophoblast attracts CD14+ cells and this frequency significantly increases in the presence of Poli:IC (p