In vitro identification and isolation of human neutrophil extracellular traps

G CALO, A TREVANI, E GRASSO, I KEITELMAN, R RAMHORST, C PÉREZ LEIRÓS AND F SABBIONE.

Detection of Cell Death Mechanisms: Methods and Protocols

Springer

Lugar: Detroit; Año: 2020;

Neutrophils release web like-structures known as neutrophil extracellular traps (NETs) that ensnare and kill microorganisms. These networks are constituted of a DNA scaffold with associated antimicrobial proteins which are released to the extracellular space as an effective mechanism to fight against invading microorganisms. In parallel with this beneficial role to avoid microbial dissemination and wall off infections, accumulating evidence supports that under certain circumstances NETs can exert deleterious effects in inflammatory, autoimmune and thrombotic pathologies. Research about NETs properties and their role in pathophysiological processes is a rapidly evolving and expanding field. Here, we describe a combination of methods to achieve a successful in vitro NETs visualization, semi-quantification and isolation.