Intracellular expression of CD69 in endometrium and peripheral blood represents an useful marker in women with recurrent miscarriage: modulation after allogeneic leukocyte immunotherapy

R. RAMHORST, V. GARCÍA, A. CORIGLIANO AND L. FAINBOIM

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY

Munsgaard

Lugar: Copenhagen; Año: 2003 vol. 49 p. 149 - 158

8755-8920

Problem: Characterize in fertile women and women with recurrent spontaneous abortions (RSA) the expression and functional status of T cells expressing the CD69 molecule. Method of study: We analyzed by flow cytometry in peripheral blood and endometrium from fertile and RSA women, the surface and cytoplasmic expression of CD69 on gated T cells. In addition, we investigated by three–color flow cytometry the expression of cytokines, and subsets of memory T cells. Results: In T cells, CD69 was restricted to the intracellular compartment with a higher frequency in RSA than in fertile women (68.2+12% vs. 23.7+22%, p