Induction of maternal tolerance to fetal alloantigens by RANTES production

R. RAMHORST. R. PATEL, A. CORIGLIANO, J.J. ETCHEPAREBORDA, L. FAINBOIM AND D. SCHUST

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY

Munksgaard

Lugar: Copenhagen; Año: 2006 vol. 56 p. 302 - 311

8755-8920

Problem: Previous sudies have demonstrated a requirement for RANTES at immune privileged sites; we have investigated the role of RANTES in the induction of maternal-fetal tolerance. Method of Study: Endometrial and peripheral T lymphocytes were obtained from women with recurrent pregnancy losses (RPL) and fertile women. RANTES modulation by progesterone or paternal alloantigens was measured by ELISA or flow cytometry analysis. Rusults: Progesterone significantly increased intracellular RANTES expression in CD4+ and CD8+ endometrial T-cells. Moreover, alloreactive lymphocytes from RPL patients produced lower RANTES-levels when compared with those from fertile women. At the local level, treatment with recombinant RANTES induced a decrease in CCR5 and CXCR4 mRNA, that correlated with an increase in T-bet expression. RPL patients and normally-fertile women express RANTES similarly, but differ in their patterns of RANTES receptor expression. Conclusions: RANTES may be implicated in the local induction of a Th1 type response necessary for successful implantation. Altered response to RANTES stimulation among some RPL patients may be responsible for poor pregnancy outcomes.