CHARACTERIZATION OF TWO SPECIFIC MICROSATELLITE SEQUENCES OF Tritrichomonas fetus

RIVERO MB; RIVERO FD; ABDALA ME; LUQUE ME; CARRANZA PG; CORIA MS; BARRIONUEVO MG; REINERI PS; DI LULLO D; LUNA BE; PALMA GA

Tafí del Valle, Tucumán

Encuentro; XXXIII ANNUAL SCIENTIFIC MEETING ABT; 2016

TUCUMAN BIOLOGY ASSOCIATION

Tritrichomonas foetus is the etiologic agent of the sexually transmitted disease limited to cattle urogenital tract known as"Bovine Trichomonosis" (TB). It is considered endemic in many countries, including Argentina, where extensive livestockwith natural service is common. TB, which causes uterine infection and abortions, is an important health problem causingsignificant economic losses. Biological intraspecies variability of TB has been poorly studied so that its knowledge couldprovide valuable information concerning its diagnosis and treatment. Microsatellite molecular markers (STR) are considereda useful tool for the study of population variability and genetics. Although the genome of T. foetus is not available yet,Trichomonas vaginalis genome, another member of the Trichomonadidae family, is available. The aim of this work was toidentify and characterize specific microsatellite sequences of T. foetus from STR characterized for T. vaginalis. From theextraction of DNA from T. foetus isolates by PCR with T. vaginalis primers, purification and sequencing of products, two STRsequences specific for T. foetus were characterized. From these sequences, specific primers for markers called STR9 andSTR10 of 277pb and 350bp respectively were designed. These tools will allow us to study variability betweenisolates withdifferent biological characteristics.