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Título:
A new appraisal on sucrose metabolism genes in microorganisms.
Autor/es:
G.L. SALERNO; M.A. KOLMAN; G. CALÓ; M. PEREZ-CENCI; C.N. NISHI
Lugar:
Tucumán- Argentina
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Resumen:
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The increasing
number of complete sequenced genomes of oxygenic photosynthetic organisms let
us to continue our study on sucrose (Suc) metabolism proteins. Searches for homologs
to sps and spp, sus and inv (genes encoding proteins of Suc
synthesis, cleavage and hydrolysis) allowed to retrieve sequences of new putative
genes. They were functionally characterized by expression in E. coli and studies of the native
proteins from cyanobacteria of the genera Synechococcus,
Thermosynechococcus, Prochorococcus, Gloebacter, Microcystis,
and Nostoc. The deduced aminoacid
sequences of the characterized SPS, SPP and SuS were used to expand our
phylogenetical analysis on Suc metabolism evolution. The presence of SuS
proteins in G. violaceus and M. aeruginosa as the unique proteins responsible
for Suc degradation allows to support the hypothesis of a distinct role for
that enzyme in unicellular than in filamentous strains. By two different lines
of evidence we conclude that the sus
genes of those strains might have been acquired by lateral gene transfer. We
conclude that whereas Suc seems to be essential in vascular plants, at least in
some photosynthetic oxygenic microrganisms it is likely to be dispensable. Gene
expression analyses suggest that its role in some microorganisms is related to environmental
conditions.