Association of VP3 to Model Membranes in Birnaviral Infection: Insights from a Computational Approach

GALASSI VANESA; M. VANINA CHIARPOTTI; LAILA SUHAIMAN; DEL PÓPOLO, MARIO; LAURA R. DELGUI

Córdoba

Congreso; LI Reunión Anual de la Sociedad Argentina de Biofísica; 2023

SAB, FCEN-UNC

The infectious bursal disease virus belongs to the Birnaviridae family, a double-stranded RNA virus group. This virus replicates using the early endosomal (EE) membrane as a platform, where the lipid phosphatidylinositol 3-phosphate (PI3P) is particularly important. At this  stage, viral protein 3 (VP3) plays a key role in membrane attachment. We address VP3 interaction with model EE membranes from a computational approach. We resorted to three distinct variants of VP3: VP3 Ct (C-terminal, residues 223 to 257, net charge +5), VP3 ΔNt (N-terminal  deletion, residues 82 to 257, electroneutral), and VP3 FL (full-length, residues 1 to 257, net charge -3). As a first step, we employed a MolecularTheory (MT) approach to obtain adsorption free-energy profiles, PMF(z), for a continuous model of an anionic membrane with 5% triacidic lipid (according to PI3P content in the EE membrane), where z is the normal distance to the interface. PMF profiles were calculated at different salt  concentrations, assessing the impact of electrostatics on the binding of  each fragment of VP3 to the membrane. VP3 Ct displayed the greatest  affinity, with -10 kT adsorption energy at the lowest salt concentration (50  M NaCl), while both VP3 ΔNt and FL binding energies were around 60%  lower. The absorption energies decreased when increasing the salt concentration, as expected from electrostatic interactions. These  estimates represent the lower bound, as all non-electrostatic interactions  are missing in MT.As a second step of our computational protocol, 500 ns Molecular Dynamics (MD) simulations based on the MARTINI model were employed  to investigate the mechanism by which VP3 binds the membrane, using VP3 Ct and VP3 ΔNt fragments. The EE membrane was modeled with a  mixture of 64:31:5 molar ratio for the lipids DOPE, DOPC (both electroneutral), and PI3P. The MD simulations revealed that both VP3 Ct  and VP3 ΔNt settled on the membrane surface immediately or after 120 ns  espectively, and remained adsorbed during the simulation. This  suggests that VP3 approaches the membrane driven by electrostatic  interactions with the VP3 Ct moiety, and an overall weak interaction withthe whole protein is sealed by a patch of four cationic residues (P2),  formed by K157, R159, H198 and R200. PI3P accumulation was observed around the VP3 Ct and P2 regions. Simulations of two different mutants, R200D and the four-fold charge-reversed P2 mutant, evidenced the role of P2 in PI3P accumulation around VP3.