DESIGN AND ANALYSIS OF AN EFFICIENT GLAUCOMA MODEL FOR EVALUATION OF PHARMACOLOGICAL TRIALS

CABALLERO, GABRIELA; ANGEL VILLEGAS, NATALIA ; DAVID CREMONEZZI; CAMPANA, VILMA; PALMA, SANTIAGO DANIEL; ALLEMANDI, DANIEL ALBERTO; TARTARA, LUIS IGNACIO

Exploratory Animal and Medical Research

West Bengal Veterinary Alumni Association

Año: 2022 vol. 12 p. 109 - 117

2277-470X

Glaucoma is a multifactorial progressive optic neuropathy whose main risk factor is intraocular hypertension (IOH). It generates loss of nerves and is the primary cause of irreversible blindness worldwide. The objective of this work was to develop a glaucoma model in rabbits and analyze the anatomical, functional and biochemical changes over time through intraocular pressure (IOP), electroretinography (ERG), antioxidant capacity with FRAP essay in aqueous humor (AH), and histopathology with quantification of retinal ganglion cells (RGC). Materials and methods: 24 female New Zealand white rabbits were used. In 12 animals, glaucoma was induced by injection of α-chymotrypsin. During the postoperative period, the treatment and control groups were examined weekly. Results: 7 days after surgery, IOP (mmHg) was 18.30 ± 1.75 in the treatment group and 13.59 ± 0.63 in the control (p