INVESTIGADORES
CABELLO Julieta Virginia
congresos y reuniones científicas
Título:
Transgenic Arabidopsis plants bearing PromoterHaHB11::HaHB11 exhibit significantly enhanced biomass
Autor/es:
GIACOMELLI JORGE I.; MORA CELESTE; CABELLO JULIETA V.; CHAN RAQUEL L.
Lugar:
Rosario, Santa Fe
Reunión:
Congreso; 50 th annual meeting Argentine society for biochemistry and molecular biology; 2014
Resumen:
HD‐Zips are plant specific transcription factors (TFs) combining a Homeobox Domain (HD) and a Leucine
Zipper (Zip). HaHB11 is a sunflower divergent HD‐Zip I TF. It was previously reported that the ectopic
and overexpression of this gene in Arabidopsis driven by the 35S constitutive promoter conferred to
transgenic plants relevant traits: tolerance to both drought and flooding stresses, larger rosettes and
improved yield compared with controls. However, this constitutive expression also caused a
developmental delay when the expression levels are very high. In order to avoid this penalty, the
HaHB11 promoter was isolated from a BAC genomic library. Transgenic plants in which this promoter
drives either the HaHB11 cDNA or the reporter GUS expression were generated and analyzed. GUS
expression was clearly detected in roots, hypocotyls and petioles of plants grown in standard conditions.
ProH11::HAHB11 plants exhibited larger rosettes than controls either in control and mild stress
conditions, significantly improved seed yield, larger roots and did not display developmental delays or
penalties. However, these plants did not show enhanced stress tolerance. In conclusion, the construct
ProH11::HAHB11 resulted in an interesting and relevant biotechnological tool to improve
biomass and yield. Further studies with enhancers or other crops would give a complete comparative
scenario.