STUDY OF ARTICULAR ADHERENTS CELLS OF TNFRp55-/- MICE WITH REACTIVE ARTHRITIS

LACOSTE, MARÍA GABRIELA; STEFANINI DE GUZMÁN, ANA MARÍA.; DI GENARO, MARÍA SILVIA

Huerta Grande, Córdoba

Congreso; I Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

Asociación de Biología de Tucumán, Sociedad Argentina de Biología, Sociedades de Biología de Cuyo, Rosario y Córdoba

Reactive arthritis (ReA) is an aseptic arthritis, which is induced by an extra-articular infection. The pathogenetic mechanism and the role of the different cellular types are unknown. The aim of the present work was to analyze the adherent cell populations derived from joints of TNFRp55-/- mice with Yersinia enterocolitica O:3 triggered-ReA. The cells were obtained from explants of inflamed joints of TNFRp55-/- mice, and cultured in DMEM at 37°C with 5% CO2. Cells were passaged when they reached confluence. The cells were observed with phase contrast and Giemsa. After 48 h, we observed round, stellate and spindle-shaped cells with prominent nucleus and nucleoli. Two weeks later, we observed multinucleated cells. The cellular morphology was consistent with macrophages and fibroblasts. After the passages, the rounded and multinucleated cells were reduced and, in a third passage, the cultures consisted almost entirely of spindle-shaped cells. We concluded that macrophages and fibroblasts are the predominant adherent cell populations in cultures from joints of mice with ReA. This methodology could be used to obtain fibroblast cultures to study the role of these cells in ReA.