CYTOKINE PROFILES IN SYNOVIAL FLUIDS OF PATIENTS WITH RHEUMATOID ARTHRITIS, SPONDYLOARTHROPATHY AND OSTEOARTHRITIS

LACOSTE, MARÍA GABRIELA; DAVE, MABEL; TAMASHIRO, HÉCTOR; BLAS, RODRIGO; LAVANCHY, ALICIA; LARREA, CLAUDIO; CAÑELLAS, ADRIANA; RUIZ, BIBIANA; RAMIS, ERICA; DI GENARO, MARÍA SILVIA

Buenos Aires

Congreso; 3er Congreso Latinoamericano de Autoinmunidad; 2010

Background and Objective: Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease. Spondyloarthropathies (SpA) are a family of related diseases which share striking points of similary such as negative rheumatoid factor. Althougth cardinal signs of inflammation in the AR and SpA joint are similar, clinical and genetic characteristics are different. Data on synovial fluid (SF) cytokine concentrations in patients with RA and SpA are limited and contradictory. The objetive of this study was compare cytokine profiles in AR, SpA and osteoarthritis (OA) patients. Methods: Interferon (IFN)-γ, Interleukin (IL)-17, IL-23(p40/19), IL-1β and IL-9 were assayed by ELISA in SF specimens from 66 AR, 17 SpA and 36 OA patients. Results: In AR, we detected significantly higher IFN-γ levels (32 pg/ml, range 5-225 pg/ml) compared with other cytokines (p< 0,05) and with the other arthropathies (p< 0,05). In SpA, pro-inflammatory cytokine levels were: IFN-γ 13 pg/ml, range 0-61 pg/ml; IL-17, 17,5 pg/ml, range 0-160 pg/ml; IL-23(p40/p19), 26 pg/ml, range 0-263 pg/ml; IL-1β, 35 pg/ml, range 0-215 pg/ml. In OA, the main proinflammatory cytokines were IL-1β (22,6 pg/ml, range 0-240 pg/ml) and IFN-γ (11 pg/ml, range 0-39 pg/ml). Interestingly, IL-17 concentrations were significantly higher in AR y SpA compared with OA (p< 0,05). In addition, correlation between IL-17 level and disease activity score (DAS) 28 was obtained in AR. IL-9 was detected in 3/56 (5.3 %), 1/15 (6.7 %) and 2/30 (6.7%) AR, SpA and OA patients, respectively. Conclusions: Cytokine profiles are clearly different in these arthropathies indicating specific pathophysiological mechanisms in these diseases.